Cucumber mosaic virus satellite RNA (Y strain): analysis of sequences which affect yellow mosaic symptoms on tobacco.

Determination of sequence and structural requirements for pathogenicity of a cucumber mosaic virus satellite RNA (Y-satRNA).

We describe the use of biologically active cDNA clones to investigate genetic determinants of a satellite RNA that modulates symptoms normally induced by its helper virus, cucumber mosaic virus (CMV). For this purpose, we have investigated a CMV satellite RNA (Y-satRNA) that induces bright yellow symptoms on tobacco and necrosis on tomato. To determine the pathogenicity-modulating domain of Y-s...

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato.

The location of a sequence within the Y satellite RNA of cucumber mosaic virus (CMV) that confers the ability to induce necrosis on tomato plants has been analysed using chimeric satellite RNAs. These recombinant RNA molecules contained parts of the Y (necrogenic) and Ra (benign) satellite RNAs and were inoculated into tomato plants together with CMV helper virus. From the composition of the re...

A Viral Satellite RNA Induces Yellow Symptoms on Tobacco by Targeting a Gene Involved in Chlorophyll Biosynthesis using the RNA Silencing Machinery

Symptoms on virus-infected plants are often very specific to the given virus. The molecular mechanisms involved in viral symptom induction have been extensively studied, but are still poorly understood. Cucumber mosaic virus (CMV) Y satellite RNA (Y-sat) is a non-coding subviral RNA and modifies the typical symptom induced by CMV in specific hosts; Y-sat causes a bright yellow mosaic on its nat...

Site-directed mutagenesis of a plant viral satellite RNA changes its phenotype from ameliorative to necrogenic.

Comparison of the nucleotide sequences of cucumber mosaic virus (CMV) satellite RNAs, which induce necrosis on tomatoes, reveals a highly conserved region within their 3' halves. The sequence of WL1 satellite (WL1-sat) RNA, which attenuates CMV symptoms on tomatoes, differs from all necrogenic satellite RNAs at three nucleotide positions within this conserved region. These nucleotides were prog...

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars